Additional Selective and Differential Cell Culture Media
NEOGEN Tryptone No. 1, 500 g
Tryptone No. 1 is an enzymatic digest of casein and meat for use in preparing microbiological culture media in a laboratory setting. Tryptone No. 1 is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone No. 1 is an enzymatic digest of casein and meat used as a nitrogen source in culture media. Casein is the main protein of milk, and a rich source of amino-acid nitrogen. Tryptone No. 1 is rich in tryptophane, making it valuable for use in detecting indole production. The absence of detectable levels of carbohydrates in Tryptone makes it a suitable peptone in differentiating bacteria on the basis of their ability to ferment various carbohydrates. Several media containing Tryptone are specified in standard methods for multiple applications.For more information visit https://www.neogen.com/solutions/Microbiology/tryptone-number-1
NEOGEN Yeast Extract, 500 g
Yeast Extract is an autolysate of yeast cells used in preparing microbiological culture media in a laboratory setting. Yeast Extract is not intended for use in the diagnosis of disease or other conditions in humans.Yeast Extract is the water-soluble portion of autolyzed yeast. The autolysis is carefully controlled to preserve naturally occurring B-complex vitamins. Yeast Extract is prepared and standardized for bacteriological use and cell cultures and is an excellent stimulator of bacterial growth. Yeast Extract is generally employed in the concentration of 0.3% - 0.5%. Yeast Extract is typically prepared by growing baker’s yeast, Saccharomyces spp., in a carbohydrate-rich plant medium. The yeast is harvested, washed, and resuspended in water, where it undergoes autolysis, i.e., self-digestion using the yeast’s enzymes.For more information visit https://www.neogen.com/solutions/Microbiology/yeast-extract
NEOGEN Sodium Chloride (Bacteriological), 5 kg
Sodium Chloride is a salt often used when preparing microbiological culture media in a laboratory setting. Sodium Chloride is not intended for use in the diagnosis of disease or other conditions in humans. When added to culture media Sodium Chloride allows for adjustments in osmotic pressure.For more information visit https://www.neogen.com/solutions/Microbiology/sodium-chloride-bacteriological
NEOGEN Lowenstein-Jensen Medium, 5 kg
Lowenstein - Jensen Medium is used with fresh egg and glycerol for the isolation and differentiation of Mycobacterium spp. in a laboratory setting. Lowenstein - Jensen Medium is not intended for use in the diagnosis of disease or other conditions in humans.The use of egg-based media for primary isolation of mycobacteria have two significant advantages. First, egg-based media support a wide variety of mycobacteria. Second, growth of mycobacteria on egg media can be used for niacin testing. Liquefaction of Lowenstein-Jensen Medium can occur if contaminated with proteolytic organisms.Lowenstein-Jensen Medium is a modification of Lowenstein Medium, modified by Jensen. Jensen modified the medium by alternating the citrate and phosphate contents, eliminating congo red dye, and increasing malachite green concentration.For more information visit https://www.neogen.com/solutions/Microbiology/lowenstein-jensen-medium
NEOGEN m-HPC Agar, 5 kg
m-HPC Agar is used for the enumeration of heterotrophic organisms in water by the membrane filtration technique in a laboratory setting. m-HPC Agar is not intended for use in the diagnosis of disease or other conditions in humans.m-HPC Agar, also known as m-Heterotrophic Plate Count Agar, was developed by Taylor and Geldreich in 1979. Monitoring the levels of heterotrophic bacteria, those microorganisms that can grow on minimal organic nutrients, is currently performed to indicate problems with treatment methods within distribution systems. There is evidence that heterotrophic bacteria are not hazardous to healthy individuals, but may be opportunistic pathogens capable of infecting individuals with impaired immune systems.m-HPC Agar is used in standard methods for the detection of heterotrophic bacteria using the membrane filtration technique.For more information visit https://www.neogen.com/solutions/Microbiology/m-hpc-agar
NEOGEN EC Medium, Modified with Novobiocin, 500 g
EC Medium, Modified with Novobiocin is used for the selective enrichment of Escherichia coli O157:H7 in a laboratory setting. EC Medium, Modified with Novobiocin is not intended for use in the diagnosis of disease or other conditions in humans.EC Medium was developed by Hajna and Perry in an effort to improve the methods for the detection of the coliform group and E. coli. This medium consists of a buffered lactose broth with the addition of 0.15% Bile Salts Mixture. Growth of spore-forming bacteria and fecal streptococci were inhibited by the bile salts.EC Medium, Modified with the addition of Novobiocin was first described by Okrend and Rose. Okrend and Rose modified EC Medium by reducing the Bile Salts Mixture concentration to 1.12% and adding 20 mg/L of sodium novobiocin.For more information visit https://www.neogen.com/solutions/Microbiology/ec-medium-modified-with-novobiocin
NEOGEN Harlequin Tryptone Bile Glucuronide Agar (TBX), 5 kg
Harlequin Tryptone Bile Glucuronide Agar (TBX) is used for the enumeration of E. coli in food and animal feed and is not intended for use in the diagnosis of disease or other conditions in humans. Tryptone Bile Glucuronide Agar is recommended by ISO 16649-1:2001, ISO 16649-2:2001 and ISO 16649-3:2015 for enumeration of β-glucuronidase positive Escherichia coli in products intended for human consumption or for the feeding of animals. The medium contains tryptone as a source of nitrogen and amino acids, bile salts act as a selective against Gram-positive organisms and agar is the gelling agent. 5-bromo-4-chloro-3-indolyl-β-D-glucuronide (X-glucuronide) is a chromogen for the detection of the β-D-glucuronidase enzyme which is present in most E. coli* but not other coliforms.*96-97% of E.coli are β-D-glucuronidase positive. A notable exception is E. coli O157:H7 For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-tryptone-bile-glucuronide-agar-tbx
NEOGEN Harlequin Cronobacter Isolation Agar, 500 g
Chromogenic Cronobacter Isolation Agar (CCI) is recommended by ISO 22964 for the isolation and identification of Cronobacter spp. (formerly Enterobacter sakazakii), and is not intended for use in the diagnosis of disease or other conditions in humans.Although rarely causing infections in immunocompetent adults, members of the Cronobacter genus have been implicated in sepsis, meningitis and necrotizing enterocolitis with a high death rate in neonates. These opportunistic pathogens are common in the environment and their ability to survive desiccation presents a significant risk for post-pasteurization contamination and survival in spray dried milk products. Tryptic digest of casein and yeast extract provide a source of nitrogen and essential vitamins. The high content of sodium chloride and the presence of sodium desoxycholate produce a selective and specific medium.For more information visit https://www.neogen.com/solutions/Microbiology/harlequin-cronobacter-isolation-agar
NEOGEN Tomato Juice Agar, 500 g
Tomato Juice Agar is used for the cultivation of lactobacilli in a laboratory setting. Tomato Juice Agar is not intended for use in the diagnosis of disease or other conditions in humans. In 1925, Mickle and Breed reported the use of tomato juice in culture media used for cultivating lactobacilli. Kulp investigated tomato juice on bacterial development and found growth of L. acidophilus was enhanced. Tomato Juice Agar, prepared according to Kulp and White’s modification, is especially useful in cultivating L. acidophilus from foodstuffs.For more information visit https://www.neogen.com/solutions/Microbiology/tomato-juice-agar
NEOGEN Brilliant Green Agar, 5 kg
Brilliant Green Agar is used for the selective isolation of Salmonella spp. in a laboratory setting. Brilliant Green Agar is not intended for use in the diagnosis of disease or other conditions in humans.Kristensen, Lester, and Jurgens first described the use of Brilliant Green Agar as a primary plating medium for the isolation of Salmonella. The report described the medium as useful for the differentiation of “paratyphoid B” from other intestinal gram-negative bacilli. Kaufmann modified their formula and used Brilliant Green Agar in addition to Tetrathionate Broth for the isolation of Salmonella from stool specimens. The outstanding selectivity of this medium permits use of moderately heavy inocula, which should be evenly distributed over the surface. Brilliant Green Agar is used in the microbial limits test. Brilliant Green Agar supplemented with novobiocin is used in food testing.For more information visit https://www.neogen.com/solutions/Microbiology/brilliant-green-agar-ncm0283
NEOGEN Acuferm Proteose Peptone #3, 10 kg
For more information visit https://www.neogen.com/solutions/microbiology/acuferm-proteose-peptone-3/
Creative Biomart Porcine Pepsin
The product is a potent enzyme in gastric juice that digests proteins such as those in meat, eggs, seeds, and dairy products.Studies on gastric digestion from 1820-1840 led to the discovery of the product as the substance which, in the presence of stomach acid, causes nutrients including meat or coagulated egg whites to dissolve. Soon afterward, it was shown that these protein nutrients were cleaved by the product to products called peptones.
Research Products International Corp Gamborg's B5 Medium, Powder, Makes 10 Liters
Basal salt mixture containing micro and macro elements as described by Gamborg, et al. (1968). Package contains 3.05 grams of powder per liter of medium.
Research Products International Corp Murashige & Skoog MS Medium, 43 Grams of Powder, Makes 10 Liters of Solution
Basal salt mixture containing micro and macro elements as described by Murashige and Skoog (1962). Concentration: 4302.09 mg/ml Hygroscopic
Research Products International Corp McCown's Woody Plant Medium, Powder, Makes 1 Liter
Basal salt mixture containing micro and macro elements as described by Lloyd & McCown (1980).